DNA Arrays

Methods and Protocols
2001
ISBN: 978-0-89603-822-6
Verlag: Humana Press

Buch, Englisch, Band 170, 268 Seiten, Format (B × H): 157 mm x 235 mm, Gewicht: 1280 g

Reihe: Methods in Molecular Biology

Microarray technology provides a highly sensitive and precise te- nique for obtaining information from biological samples, with the added advantage that it can handle a large number of samples simultaneously that may be analyzed rapidly. Researchers are applying microarray technology to understand gene expression, mutation analysis, and the sequencing of genes. Although this technology has been experimental, and thus has been through feasibility studies, it has just recently entered into widespread use for advanced research. The purpose of DNA Arrays: Methods and Protocols is to provide instruction in designing and constructing DNA arrays, as well as hybridizing them with biological samples for analysis. An additional purpose is to p- vide the reader with a broad description of DNA-based array technology and its potential applications. This volume also covers the history of DNA arrays—from their conception to their ready off-the-shelf availability—for readers who are new to array technology as well as those who are well versed in this field. Stepwise, detailed experimental procedures are described for constructing DNA arrays, including the choice of solid support, attachment methods, and the general conditions for hybridization. With microarray technology, ordered arrays of oligonucleotides or other DNA sequences are attached or printed to the solid support using au- mated methods for array synthesis. Probe sequences are selected in such a way that they have the appropriate sequence length, site of mutation, and T.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Rampal, Jang B.

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Biomedizin, Medizinische Forschung, Klinische Studien
• Naturwissenschaften Biowissenschaften Biowissenschaften DNA und Transgene Organismen
• Naturwissenschaften Biowissenschaften Zellbiologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Humangenetik

Inhaltsverzeichnis

DNA Microarrays.- Gel-Immobilized Microarrays of Nucleic Acids and Proteins.- Sequencing by Hybridization Arrays.- Ethical Ramifications of Genetic Analysis Using DNA Arrays.- Photolithographic Synthesis of High-Density Oligonucleotide Arrays.- Automated Genotyping Using the DNA MassArray™ Technology.- Ink-Jet-Deposited Microspot Arrays of DNA and Other Bioactive Molecules.- Printing DNA Microarrays Using the Biomek® 2000 Laboratory Automation Workstation.- Hybridization Analysis of Labeled RNA by Oligonucleotide Arrays.- Analysis of Nucleic Acids by Tandem Hybridization on Oligonucleotide Microarrays.- DNA Sequencing by Hybridization with Arrays of Samples or Probes.- Using Oligonucleotide Scanning Arrays to Find Effective Antisense Reagents.- Low-Resolution Typing of HLA-DQA1 Using DNA Microarray.- Gene Expression Analysis on Medium-Density Oligonucleotide Arrays.- Use of Bioinformatics in Arrays.- Confocal Scanning of Genetic Microarrays.- Business Aspects of Biochip Technologies.