Buch, Englisch, 380 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 750 g
Buch, Englisch, 380 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 750 g
ISBN: 978-0-323-90737-8
Verlag: William Andrew Publishing
Additional sections cover the Use of native mass spectrometry to guide detergent-based rescue of non-native oligomerization by recombinant proteins, Advancing overexpression and purification of recombinant proteins by pilot optimization through tandem affinity-buffer exchange chromatography online with native mass spectrometry, Method for High-Efficiency Fed-batch cultures of recombinant Escherichia coli, Method to transfer Chinese hamster ovary (CHO) shake flask experiments to the ambr® 250, and Expression of recombinant antibodies in Leishmania tarentolae.
Zielgruppe
<p>Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists</p>
Fachgebiete
Weitere Infos & Material
Preface
1. Expression of recombinant multi-protein complexes in Saccharomyces cerevisiae
2. Recombinant production of membrane proteins in yeast
3. Expression of proteins in Pichia pastoris
4. Hybrid-architectured promoter design to engineer expression in yeast
5. Hybrid-architectured promoter design to deregulate expression in yeast
Baculovirus-insect cell systems
6. The MultiBac BEVS: Basics, Applications, Performance and Recent Developments
7. Applications of Golden Gate Cloning to protein production using the baculovirus expression vector system
8. A fast-track protocol for protein expression using the BEV system
Plant hosts
9. Transient expression of recombinant proteins in plants
10. Transient expression of recombinant proteins in plants using potato virus X based vectors
11. Design and expression of a bispecific antibody against dengue and chikungunya virus in plants
12. Manufacturing plant-made monoclonal antibodies for research or therapeutic applications
Mammalian cell lines as biomanufacturing platforms
13. Creation of monoclonal antibody expressing CHO cell lines grown with sodium butyrate and characterization of resulting antibody glycosylation
14. Method to transfer Chinese hamster ovary (CHO) batch shake flask experiments to large-scale, computer-controlled fed-batch bioreactors
15. Inducible protein expression in piggyBac transposase mediated stable HEK293 cell pools
16. Exploring the design space of AAV transient-transfection in suspension cell lines