Ricke / Park / Davis Microbial Transposon Mutagenesis

Protocols and Applications
Erscheinungsjahr 2019
ISBN: 978-1-4939-9570-7
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 2016, 183 Seiten, eBook

Reihe: Methods in Molecular Biology

This book addresses cutting-edge techniques for researching transposon mutagenesis, an approach for identifying individual gene contributions to the phenotypic characteristics of a particular microorganism. The volume begins with methods for specific microorganisms and include protocols for individual microorganisms ranging from pathogens such as

Salmonella

to

Bifidobacterium

, a microorganism considered beneficial to humans and animals. The final section addresses more general protocols including plasmid transfer and bioinformatic tools as well as novel applications of transposon methodologies such as transposon-aided capture of antibiotic resistant plasmids. Written for the highly successful

Methods in Molecular Biology

series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical,

MicrobialTransposon Mutagenesis: Protocols and Applications

serves as a valuable reference for scientists seeking to apply transposon mutagenesis to microbial genetic analyses and functionality.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Ricke, Steven C.

Park, Si Hong

Davis, Morgan L.

Inhaltsverzeichnis

Preparation of Transposon Library and Tn-Seq Amplicon Library for
Salmonella
Typhimurium.- Application of Whole Genome Sequencing to Transposon Mutants of
Salmonella
Heidelberg.- Construction of a Sequence-Defined Transposon Mutant Library in 
Staphylococcus aureus
.- Transposon Mutagenesis in
Streptococcus
Species.- Implementation of Transposon Mutagenesis in
Bifidobacterium
.- Transposon Mutagenesis of
Listeria monocytogenes
.- Transposon Mutagenesis of Foodborne Pathogenic
Esherichia coli
.- Methods for Transposon Mutagenesis in
Proteus mirabilis.- 
Mutagenesis of 
Vibrio fischeri
 and Other Marine Bacteria Using Hyperactive Mini-Tn
5
Derivatives.- Transposon Mutagenesis of
Bacteroides fragilis
.- Transposon Mutagenesis in
Mycobacterium avium
Subspecies
Paratuberculosis.- 
Protocols of Conjugative Plasmid Transfer in
Salmonella
: Plate, Broth, and Filter Mating Approaches.- Construction of DNA Barcode-Tagged
Salmonella
Strains.- Transposon-Aided Capture of Antibiotic Resistance Plasmids from Complex Samples.- Efficient Gene Deletion Method for
Listeria monocytogenes
.- Whole Genome Identification and Characterization of Bacterial Insertion Sequences Using Bioinformatic Tools.