Chiral Separations

Methods and Protocols
1. Auflage. Softcover version of original hardcover Auflage 2004
ISBN: 978-1-61737-390-9
Verlag: Humana Press

Buch, Englisch, Band 243, 432 Seiten, Previously published in hardcover, Format (B × H): 155 mm x 235 mm, Gewicht: 756 g

Reihe: Methods in Molecular Biology

Many compounds of biological and pharmacological interest are as- metric and show optical activity. Approximately 40% of the drugs in use are known to be chiral and only about 25% are administered as pure enantiomers. It is well established that the pharmacological activity is mostly restricted to one of the enantiomers (eutomer). In several cases, unwanted side effects or even toxic effects may occur with the inactive enantiomer (distomer). Even if the side effects are not that drastic, the inactive enantiomer has to be meta- lized, which represents an unnecessary burden for the organism. The admin- tration of pure, pharmacologically active enantiomers is therefore of great importance. The ideal way to get to pure enantiomers would be by enantioselective synthesis. However, this approach is usually expensive and not often practicable. Usually, the racemates are obtained in a synthesis, and the separation of the enantiomers on a preparative scale is necessary. On the other hand, there is also a great demand for methods of enantiomer separation on an analytical scale for controlling synthesis, checking for racemization p- cesses, controlling enantiomeric purity, and for pharmacokinetic studies. C- ventional methods for enantiomer separation on a preparative scale are fractionated crystallization, the formation of diastereomeric pairs followed by repeated recrystallization, and enzymatic procedures. In recent years, ch- matographic methods such as gas chromatography and, especially, liquid ch- matography have attracted increasing interest for chiral separation, both on analytical and preparative scales.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Schmid, Martin G.

Gübitz, Gerald

Fachgebiete

• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Chemie Analytische Chemie Instrumentelle Analytik, Chromatographie
• Naturwissenschaften Chemie Organische Chemie Biochemie

Inhaltsverzeichnis

Chiral Separation Principles.- Separation of Enantiomers by Thin-Layer Chromatography.- Cyclodextrin-Based Chiral Stationary Phases for Liquid Chromatography.- Enantiomeric Separations by HPLC Using Macrocyclic Glycopeptide-Based Chiral Stationary Phases.- Chiral Separation by HPLC Using Polysaccharide-Based Chiral Stationary Phases.- Applications of Polysaccharide-Based Chiral Stationary Phases for Resolution of Different Compound Classes.- Chiral Separation by HPLC With Pirkle-Type Chiral Stationary Phases.- Chiral Separation by HPLC Using the Ligand-Exchange Principle.- Chiral Separations by HPLC Using Molecularly Imprinted Polymers.- Indirect Enantioseparation by HPLC Using Chiral Benzofurazan-Bearing Reagents.- Separation of Racemic Trans-Stilbene Oxide by Sub-/Supercritical Fluid Chromatography.- Chiral Separations Using the Macrocyclic Antibiotics in Capillary Electrophoresis.- Enantioresolutions by Capillary Electrophoresis Using Glycopeptide Antibiotics.- Separation of Enantiomers by Capillary Electrophoresis Using Cyclodextrins.- Chiral Separations by Capillary Electrophoresis Using Proteins as Chiral Selectors.- Cellulases as Chiral Selectors in Capillary Electrophoresis.- Use of Chiral Crown Ethers in Capillary Electrophoresis.- Chiral Separations by Capillary Electrophoresis Using Cinchona Alkaloid Derivatives as Chiral Counter-Ions.- Chiral Separation by Capillary Electrophoresis Using Polysaccharides.- Chiral Micellar Electrokinetic Chromatography.- Chiral Separation by Capillary Electrophoresis in Nonaqueous Medium.- Chiral Ligand-Exchange Capillary Electrophoresis and Capillary Electrochromatography.- Enantioseparation in Capillary Chromatography and Capillary Electrochromatography Using Polysaccharide-Type Chiral Stationary Phases.- Chiral Separation by Capillary Electrochromatography Using Cyclodextrin Phases.- Chiral Separations by Capillary Electrochromatography Using Molecularly Imprinted Polymers.