The Immunological Synapse - Part B

Erscheinungsjahr 2023
ISBN: 978-0-443-19163-3
Verlag: Elsevier Science & Technology

Buch, Englisch, 318 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 630 g

The Immunological Synapse - Part B, Volume 178 in the Methods in Cell Biology series provides state-of-the-art methods for the study of the immunological synapse. This first volume covers various aspects on T cell and natural killer (NK) cell synapses, including imaging polarized granule release using TIRF microscopy, analysis of actin reorganization and centrosome polarization, redirected degranulation, live cell-imaging to quantify cytotoxic and chemotactic dynamics, quantification of interactions between APCs and T cells, assessment of membrane lipid state at the immunological synapse, proteomic analysis and imaging of NK-tumor cell interaction, evaluating natural killer cell effector functions against breast cancer cells derived from human tumor tissue, evaluation of chimeric antigen receptor (CAR)-modified cell immunological synapse quality using the glass-supported planar lipid bilayer, and monitoring potency of therapeutic CAR T cells. It also includes one chapter on quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers, and one chapter on the murine antibody-dependent cellular phagocytosis assay.

Zielgruppe

Scientists and researchers

Autoren/Hrsg.

Weitere Mitwirkende

Thomas, Clément

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Entwicklungsbiologie
• Naturwissenschaften Biowissenschaften Zellbiologie

Inhaltsverzeichnis

Contributors in this volume include:

Torsten Tonn
Jiri Eitler
Rose Thierry
Clément Thomas
Jacques Zimmer
Carsten Watzl
Pedro Proda
Arnika Kathleen Wagner
Alicia Copik
Claudio Giraudo
Loic Dupre
Felix Borgmann
Chenguang Xu
Wanli Liu

Über Autor(innen)

Thomas, Clément
Clément Thomas, Ph.D. is a research group leader at the Department of Cancer Research of the Luxembourg Institute of Health, Luxembourg. He received his Ph.D. in Molecular and Cellular Biology from the University of Strasbourg. Over the last 15 years, his group has developed extensive knowledge and expertise in the mechanisms regulating actin cytoskeleton organization and dynamics, as well as in live cell imaging approaches. His current research is centered on the role of the actin cytoskeleton in tumor immune evasion.