Two-Component Signaling Systems, Part B

Erscheinungsjahr 2007
ISBN: 978-0-12-373852-3
Verlag: William Andrew Publishing

Buch, Englisch, 648 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 1330 g

Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system."

Sections in Two-Component Signaling Systems, Part B include:

- Structural Approaches
- Reconstitution of Heterogeneous Systems
- Intracellular Methods and Assays
- Genome-Wide Analyses of Two-Component Systems

Zielgruppe

Biochemists, geneticists, and molecular biologists.

Autoren/Hrsg.

Weitere Mitwirkende

Simon, Melvin I.

Crane, Brian

Crane, Alexandrine

Fachgebiete

• Naturwissenschaften Biowissenschaften Enzymologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen

Inhaltsverzeichnis

Section I: Structural Approaches

[1]: The PICM Chemical Scanning Method for Identifying Domain-Domain and Protein-Protein Interfaces: Applications to the Core Signaling Complex of E. coli Chemotaxis

[2]: Use of Site-Directed Cysteine and Disulfide Chemistry to Probe Protein Structure and Dynamics: Applications to Soluble and Transmembrane Receptors of Bacterial Chemotaxis

[3]: Measuring Distances by Pulsed Dipolar ESR Spectroscopy: Spin-Labeled Histidine Kinases

[4]: Rigid Body Refinement of Protein Complexes with Long-Range Distance Restraints from Pulsed Dipolar ESR

[5]: TonB/TolA Amino-Terminal Domain Modeling

[6]: Functional Dynamics of Response Regulators Using NMR Relaxation Techniques

[7]: The Design and Development of Tar-EnvZ Chimeric Receptors

[8]: Functional and Structural Characterization of EnvZ, an Osmosensing Histidine Kinase of E. coli

[9]: Light Modulation of Histidine-Kinase Activity in Bacterial Phytochromes Monitored by Size Exclusion Chromatography, Crosslinking, and Limited Proteolysis

[10]: A Temperature-Sensing Histidine Kinase-Function, Genetics, and Membrane Topology

[11]: The Regulation of Histidine Sensor Kinase Complexes by Quorum Sensing Signal Molecules

Section II: Reconstitution of Heterogeneous Systems

[12]: Liposome-Mediated Assembly of Receptor Signaling Complexes

[13]: Analyzing Transmembrane Chemoreceptors Using In Vivo Disulfide Formation Between Introduced Cysteines

[14]: Using Nanodiscs to Create Water-Soluble Transmembrane Chemoreceptors Inserted in Lipid Bilayers

[15]: Assays for CheC, FliY, and CheX as Representatives of Response Regulator Phosphatases

[16]: Genetic Dissection of Signaling Through the Rcs Phosphorelay

Section III: Intracellular Methods and Assays

[17]: In Vivo Measurement by FRET of Pathway Activity in Bacterial Chemotaxis

[18]: In Vivo and In Vitro Analysis of the Rhodobacter sphaeroides Chemotaxis Signaling Comp