Uppu / Murthy / Pryor Free Radicals and Antioxidant Protocols

2. Auflage 2010
ISBN: 978-1-60327-029-8
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 610, 480 Seiten, eBook

Reihe: Methods in Molecular Biology

Zielgruppe

Research

Autoren/Hrsg.

Uppu, Rao M.

Murthy, Subramanyam N.

Pryor, William A.

Parinandi, Narasimham L.

Inhaltsverzeichnis

Reactive Oxygen and Nitrogen Species.- In Vivo Imaging of Free Radicals and Oxygen.- In Vivo Measurement of Tissue Oxygen Using Electron Paramagnetic Resonance Spectroscopy with Oxygen-Sensitive Paramagnetic Particle, Lithium Phthalocyanine.- Measurement of Plasma Nitrite by Chemiluminescence.- Determination of Glutathione, Mitochondrial Transmembrane Potential, and Cytotoxicity in H9c2 Cardiomyoblasts Exposed to Reactive Oxygen and Nitrogen Species.- Natural and Synthetic Antioxidants.- Phenolic Acids and Flavonoids: Occurrence and Analytical Methods.- Design, Synthesis, and Action of Antiatherogenic Antioxidants.- Preparation of Drug-Loaded Polymeric Nanoparticles and Evaluation of the Antioxidant Activity Against Lipid Peroxidation.- Nanoparticle and Iron Chelators as a Potential Novel Alzheimer Therapy.- Synthesis and Characterization of Polymer Nanocarriers for the Targeted Delivery of Therapeutic Enzymes.- Assessment of Antioxidant Activity of Eugenol In Vitro and In Vivo.- Cellular Oxidative Stress.- The Generation of Stable Oxidative Stress-Resistant Phenotypes in Chinese Hamster Fibroblasts Chronically Exposed to Hydrogen Peroxide or Hyperoxia.- A Simple Method for Effective and Safe Removal of Membrane Cholesterol from Lipid Rafts in Vascular Endothelial Cells: Implications in Oxidant-Mediated Lipid Signaling.- Superoxide Dismutase – A Target for Gene Therapeutic Approach to Reduce Oxidative Stress in Erectile Dysfunction.- Assessing the Reductive Capacity of Cells by Measuring the Recycling of Ascorbic and Lipoic Acids.- Biomarkers of Oxidative Stress: Methods and Measures of Oxidative DNA Damage (COMET Assay) and Telomere Shortening.- Simultaneous Analysis of Expression of Multiple Redox-Sensitive and Apoptotic Genes in Hypothalamic Neurons Exposed toCholesterol Secoaldehyde.- Redox Homeostasis and Cellular Stress Response in Aging and Neurodegeneration.- Gene Therapy Techniques for the Delivery of Endothelial Nitric Oxide Synthase to the Lung for Pulmonary Hypertension.- DNA Oxidation, Oxidative Lipidomics, and Biomarkers.- A General Method for Quantifying Sequence Effects on Nucleobase Oxidation in DNA.- Analysis of Urinary 8-oxo-7,8-dihydro-2?-deoxyguanosine by Liquid Chromatography–Tandem Mass Spectrometry.- Oxidative Lipidomics of Apoptosis: Quantitative Assessment of Phospholipid Hydroperoxides in Cells and Tissues.- Simultaneous Analysis of Multiple Lipid Oxidation Products In Vivo by Liquid Chromatographic-Mass Spectrometry (LC-MS).- Lipoxygenase-Catalyzed Phospholipid Peroxidation: Preparation, Purification, and Characterization of Phosphatidylinositol Peroxides.- Oxidized Low-Density Lipoprotein.- Detection and Localization of Markers of Oxidative Stress by In Situ Methods: Application in the Study of Alzheimer Disease.- Enzyme Immunoassay of Isoprostanes.- Application of Membrane Extraction with Sorbent Interface for Breath Analysis.