Free Radicals and Antioxidant Protocols

Softcover Nachdruck of hardcover 2. Auflage 2010
ISBN: 978-1-61779-683-8
Verlag: Humana Press

Buch, Englisch, Band 610, 480 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 924 g

Reihe: Methods in Molecular Biology

Reactive oxygen and nitrogen species (RONs) affect normal physiological processes and pathological conditions. In spite of the development of analytical and technological advancements in the detection and determination of RONs, there is a growing demand to achieve more rapid and accurate detection and determination of RONs and also of redox stress. In order to satisfy such a demand, we chose to prepare this current volume which comprises 27 chapters contributed by world-renowned experts on the state-- the-art analytical and technological aspects of the detection and determination of RONs, and oxidative, nitrative, nitrosative, and redox stresses in biological systems, in vitro and in vivo. There is a need to establish biomarkers for the oxidative stress-induced genetic d- age in cellular and animal models. This need is addressed herein by offering protocols for (1) the generation of stable oxidative stress-resistant phenotypes of Chinese hamsters ?broblasts, (2) in vivo detection and measurement of free radicals and oxygen, (3) in vivo determination of tissue and DNA damage as a result of radical exposure, and (4) in vivo and in vitro monitoring of footprints of free-radical and antioxidant reactions. In the s- tions dedicated to the analysis of antioxidants and their metabolites, we describe methods for the analysis of phenolic acids and ?avonoids, eugenol antioxidants, and the recycling of ascorbic and lipoic acids. We also report on cellular reductive capacity, determination of glutathione, mitochondrial transmembrane potential, and cytotoxicity in cardiomyocytes under RONs stresses.

Zielgruppe

Research

Autoren/Hrsg.

Uppu, Rao M.

Parinandi, Narasimham L.

Murthy, Subramanyam N.

Pryor, William A.

Fachgebiete

• Naturwissenschaften Chemie Organische Chemie Biochemie
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Chemie Analytische Chemie

Inhaltsverzeichnis

Reactive Oxygen and Nitrogen Species.- In Vivo Imaging of Free Radicals and Oxygen.- In Vivo Measurement of Tissue Oxygen Using Electron Paramagnetic Resonance Spectroscopy with Oxygen-Sensitive Paramagnetic Particle, Lithium Phthalocyanine.- Measurement of Plasma Nitrite by Chemiluminescence.- Determination of Glutathione, Mitochondrial Transmembrane Potential, and Cytotoxicity in H9c2 Cardiomyoblasts Exposed to Reactive Oxygen and Nitrogen Species.- Natural and Synthetic Antioxidants.- Phenolic Acids and Flavonoids: Occurrence and Analytical Methods.- Design, Synthesis, and Action of Antiatherogenic Antioxidants.- Preparation of Drug-Loaded Polymeric Nanoparticles and Evaluation of the Antioxidant Activity Against Lipid Peroxidation.- Nanoparticle and Iron Chelators as a Potential Novel Alzheimer Therapy.- Synthesis and Characterization of Polymer Nanocarriers for the Targeted Delivery of Therapeutic Enzymes.- Assessment of Antioxidant Activity of Eugenol In Vitro and In Vivo.- Cellular Oxidative Stress.- The Generation of Stable Oxidative Stress-Resistant Phenotypes in Chinese Hamster Fibroblasts Chronically Exposed to Hydrogen Peroxide or Hyperoxia.- A Simple Method for Effective and Safe Removal of Membrane Cholesterol from Lipid Rafts in Vascular Endothelial Cells: Implications in Oxidant-Mediated Lipid Signaling.- Superoxide Dismutase – A Target for Gene Therapeutic Approach to Reduce Oxidative Stress in Erectile Dysfunction.- Assessing the Reductive Capacity of Cells by Measuring the Recycling of Ascorbic and Lipoic Acids.- Biomarkers of Oxidative Stress: Methods and Measures of Oxidative DNA Damage (COMET Assay) and Telomere Shortening.- Simultaneous Analysis of Expression of Multiple Redox-Sensitive and Apoptotic Genes in Hypothalamic Neurons Exposed toCholesterol Secoaldehyde.- Redox Homeostasis and Cellular Stress Response in Aging and Neurodegeneration.- Gene Therapy Techniques for the Delivery of Endothelial Nitric Oxide Synthase to the Lung for Pulmonary Hypertension.- DNA Oxidation, Oxidative Lipidomics, and Biomarkers.- A General Method for Quantifying Sequence Effects on Nucleobase Oxidation in DNA.- Analysis of Urinary 8-oxo-7,8-dihydro-2?-deoxyguanosine by Liquid Chromatography–Tandem Mass Spectrometry.- Oxidative Lipidomics of Apoptosis: Quantitative Assessment of Phospholipid Hydroperoxides in Cells and Tissues.- Simultaneous Analysis of Multiple Lipid Oxidation Products In Vivo by Liquid Chromatographic-Mass Spectrometry (LC-MS).- Lipoxygenase-Catalyzed Phospholipid Peroxidation: Preparation, Purification, and Characterization of Phosphatidylinositol Peroxides.- Oxidized Low-Density Lipoprotein.- Detection and Localization of Markers of Oxidative Stress by In Situ Methods: Application in the Study of Alzheimer Disease.- Enzyme Immunoassay of Isoprostanes.- Application of Membrane Extraction with Sorbent Interface for Breath Analysis.