Varma Mycorrhiza Manual

Erscheinungsjahr 2012
ISBN: 978-3-642-60268-9
Verlag: Springer
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 542 Seiten, eBook

Reihe: Springer Lab Manuals

Mycorrhiza - symbiotic associations between plant roots and fungi - play a major role in many fundamental plant functions such as mineral nutrition or stress resistance. As the link between plants and the soil, mycorrhiza are now of great interest for developing new strategies in sustainable agriculture. Since they allow a decreased use of fertilizer and pesticides, negative impacts on the environment can be minimized. With contributions from renowned international scientists, this manual offers a great variety of practical protocols for analyzing mycorrhiza, including the latest molecular, biochemical, genetical, and physiological techniques.

Zielgruppe

Research

Autoren/Hrsg.

Varma, Ajit

Inhaltsverzeichnis

1 Mycorrhizae, the Friendly Fungi: What We Know, What Should We Know, and How Do We Know?.- 2 Approaches to the Study of Interactions Between Arbuscular Mycorrhizal Fungi.- 3 Application of Molecular Techniques to Ecological Studies of Symbioses in Actinorhizal Plants.- 4 Characterizing Effects of Mycorrhizal Fungi on Plant Population Structure.- 5 Evaluation of Vesicular-Arbuscudar Mycorrhizal Colonisation by Staining.- 6 Mycorrhizosphere Microorganisms: Screening and Evaluation.- 7 Methods for Cultivating Edible Ectomycorrhizal Mushrooms.- 8 Isolation and Regeneration of Protoplasts from Ectomycorrhizal Fungi.- 9 Electrophoretic Analytical Technique for the Genetic Identification of Mycorrhizal Fungi of the Genus Tuber and Their Mycelium and Mycorrhiza.- 10 The Use of Polytetrafluoroethylene (PTFE) Hydrophobic Membranes to Study Transport of N15 by Mycorrhizal Hyphae.- 11 Application of Methods of Quantitative Histochemistry on Mycorrhizal Roots.- 12 Measuring Production and Activity of Plant Cell Wall-Degrading Enzymes in Ectomycorrhizal Fungi.- 13 Biochemical Analysis of Polygalacturonases by Ericoid Mycorrhizal Fungi.- 14 Methods for Determining Extracellular and Surface-Bound Phosphatase Activities in Ectomycorrhizal Fungi.- 15 Determination of Glutathione, Glutathione Disulphide and Two Related Enzymes — Glutathione Reductase and Glucose-6-Phosphate Dehydrogenase, in Fungal and Plant Cells.- 16 Identification and Characterzation of Siderophores of Mycorrhizal Fungi.- 17 Polyclonal Antibodies for the Detection of Arbuscular Mycorrhizal Fungi.- 18 Monoclonal Antibodies for the Identification of Arbuscular Mycorrhizal Fungi.- 19 Targeted Approaches for Detecting Changes in Protein Expression with Mycorrhiza Development: Hydrolytic Isoenzyme Analyses andImmunological Detection of Known Proteins in Root Extracts.- 20 Untargeted Approaches to the Analysis of Gene Expression in Arbuscular Mycorrhiza: Analyses of Polypeptides (in Vivo and in Vitro) and of Translated mRNA Products.- 21 The Molecular Route to Understanding VAM Symbiosis.- 22 Molecular Approaches to Investigate Biodiversity in Mycorrhizal Fungi.- 23 DNA Isolation and Development of Molecular Tools for Identification and Detection of VA Mycorrhizal Fungi.- 24 PCR in Studies of AM Fungi: from Primers to Application.- 25 PCR Cloning of Genes from Arbuscular Mycorrhizal Fungi.- 26 RAPD and ITS Analysis of Orchid Mycorrhizal Fungi.- 27 Isolation and Partial Characterisation of Double-Stranded RNA-Containing Viruses of Orchid Mycorrhizal Fungi.- 28 Identification and Characterization of Genes by Mutant Complementation.- 29 Spatial Regulation of Genes in Ectomycorrhiza: mRNA in Situ Localization and ?-Glucuronidase Detection.- 30 Genomic Fingerprinting of Ectomycorrhizal Fungi by Microsatellite-primed PCR.- 31 Large-Scale Sequencing of Anonymous Ectomycorrhiza cDNA Clones.- 32 Production of Biomass of Arbuscular Mycorrhizal Fungi in the Glass Bead Compartment System.- 33 DNA Amplification Fingerprinting of Mycorrhizal Fungi and Associated Plant Materials Using Arbitrary Primers.- 34 Quantification of AMF-Induced Modifications to Root System Architecture and Longevity.