Vaschetto Cereal Genomics

Methods and Protocols
Erscheinungsjahr 2019
ISBN: 978-1-4939-9865-4
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 2072, 247 Seiten, eBook

Reihe: Methods in Molecular Biology

The objective of this volume is to detail current technologies associated with cereal genomics, providing a valuable resource for researchers working in breeding and molecular crop improvement programs. Chapters guide readers through high-throughput DNA extraction protocols, crop genetic resources, meta-Quantitative Trait Loci (QTL) analysis, association mapping, next-sequencing generation, transposable element-associated variation, transcriptomics analysis, epigenetic variation, identification of imprinted genes, noncoding RNAs (circular RNAs), genome editing technologies, and post-translational protein phosphorylation.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Cereal Genomics: Methods and Protocols to ensure successful results in the further study of this vital field.

Zielgruppe

Popular/general

Autoren/Hrsg.

Vaschetto, Luis M.

Inhaltsverzeichnis

A Low-Cost High-throughput Method for Plant Genomic DNA Isolation.- Cereal Genomics Databases and Plant Genetic Resources in Crop Improvement.- Integrated Genomic Strategies for Cereal Genetic Enhancement: Combining QTL and Association Mapping.- Sequencing and Assembling Genomes and Chromosomes of Cereal Crops.- Cost-effective Profiling of
Mutator
Transposon Insertions in Maize by Next-generation Sequencing.- Relative Expression Analysis of target genes by using Reverse transcription-Quantitative PCR.- DNA Methylation and Transcriptomic Next Generation Technologies in Cereal Genomics.- Genome-wide Identification of Regulatory DNA Elements in Crop Plants.- Genome-wide Profiling of Histone Modifications with ChIP-seq.- Whole-genome Bisulfite Sequencing and Epigenetic Variation in Cereal Methylomes.- Genome-wide Identification of Allele-Specific Gene Expression in a Parent-of-Origin Specific Manner.- Analysis of Epigenetic Modifications during Vegetative and Reproductive Development in Cereals using Chromatin Immunoprecipitation (ChIP).- Cereal Circular RNAs (circRNAs): An Overview of the Computational Resources for Identification and Analysis.- Emerging Genome Engineering Tools in Crop Research and Breeding.- CRISPR/Cas9-mediated Targeted Mutagenesis in Wheat Doubled Haploids.- Genetic Transformation of Protoplasts Isolated from Leaves of
Lolium temulentum
and
Lolium perenne
.- 
Agrobacterium
Transformation in the Rice Genome.- 
In vivo
Phosphorylation: Development of Specific Antibodies to Detect the Phosphorylated PEPC Isoform for the C
4
Photosynthesis in
Zea mays.