Walker Basic Protein and Peptide Protocols

Erscheinungsjahr 2008
ISBN: 978-1-59259-519-8
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 32, 490 Seiten, eBook

Reihe: Methods in Molecular Biology

Zielgruppe

Research

Autoren/Hrsg.

Walker, John M.

Inhaltsverzeichnis

The Lowry Method for Protein Quantitation.- The Bicinchoninic Acid (BCA) Assay for Protein Quantitation.- The Bradford Method for Protein Quantitation.- Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins.- SDS Polyacrylamide Gel Electrophoresis of Proteins.- Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins.- Acetic Acid-Urea Polyacrylamide Gel Electrophoresis of Proteins.- Electrophoresis of Peptides.- Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels.- The In Vivo Isotopic Labeling of Proteins for Polyacrylamide Gel Electrophoresis.- Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins.- Two-Dimensional Polyacrylamide Gel Electrophoresis Using Immobilized pH Gradients in the First Dimension.- Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the First Dimension.- Quantification of Proteins on Polyacrylamide Gels (Nonradioactive).- Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique.- Identification of Glycoproteins on Nitrocellulose Membranes and Gels.- The Release of Oligosaccharides from Glycoproteins.- Structural Profiling of Oligosaccharides of Glycoproteins.- Drying Gels.- Fluorography of Polyacrylamide Gels Containing Tritium.- The Electrophoretic Elution of Proteins from Polyacrylamide Gels.- Preparation of Proteins from Gels for Protein Microsequencing.- Comparison of Protein Primary Structures.- Protein Blotting.- Detection of Polypeptides on Immunoblots Using Secondary Antibodies or Protein A.- Detection of Proteins on Blots Using the Avidin-Biotin System.- Detection of Proteins on Protein Blots Using Chemiluminescent Systems.- The Detection of Proteins on Blots Using Gold or Immunogold.- Amino Acid Analysis.- Molecular-Weight Estimation for Native Proteins Using Size-Exclusion High-Performance Liquid Chromatography.- Peptide Purification by Reverse-Phase HPLC.- Enzymatic Methods for Cleaving Proteins.- Chemical Cleavage of Proteins.- Chemical Modification of Proteins.- The Dansyl Method for Identifying N-Terminal Amino Acids.- The Dansyl-Edman Method for Peptide Sequencing.- C-Terminal Sequencing of Peptides.- Analysis of Cysteine Residues and Disulfide Bonds.- Preparation and Characterization of Monoclonal Antibodies to Proteins and Other Cellular Components.- The Raising of a Polyclonal Antiserum to a Protein.- Production of Antisera to Synthetic Peptides.- Production of Antibodies Using Proteins in Gel Bands.- Preparation of Purified Immunoglobulin G (IgG).- Enzyme and Biotin Labeling of Antibody.- Labeling of Peptides and Proteins by Radioiodination.- Radioimmunoassay of Peptides and Proteins.- Enzyme-Linked Immunosorbent Assay (ELISA).- Free Zone Capillary Electrophoresis.