Confocal Microscopy

Methods and Protocols
1. Auflage 1998
ISBN: 978-0-89603-526-3
Verlag: Humana Press

Buch, Englisch, Band 122, 464 Seiten, Format (B × H): 155 mm x 235 mm, Gewicht: 938 g

Reihe: Methods in Molecular Biology

Stephen Paddock and a highly skilled panel of experts lead the researcher using confocal techniques from the bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, nontechnical style, the methods specifically cover most of the commonly used model organisms: worms, sea urchins, flies, plants, yeast, frogs, and zebrafish. The powerful hands-on methods collected here will help even the novice to produce first-class cover-quality confocal images.

Zielgruppe

Research

Autoren/Hrsg.

Paddock, Stephen W.

Fachgebiete

• Naturwissenschaften Physik Elektromagnetismus Mikroskopie, Spektroskopie
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)

Inhaltsverzeichnis

An Introduction to Confocal Imaging, Stephen W. Paddock. Practical Considerations for Collecting Confocal Images, David Carter. Fluorescent Probes for Confocal Microscopy, Christopher Cullander. Imaging Gene Expression Using Antibody Probes, Nadean L. Brown. Single and Double FISH Protocols for Drosophila, Sarah C. Hughes and Henry M. Krause. Confocal Microscopy of Plant Cells, Carol L. Wymer, Alison F. Bevan, Kurt Boudonck, and Clive W. Lloyd. Preparation of Yeast Cells for Confocal Microscopy, Audrey Atkin. Confocal Methods for C. elegans, Sarah L. Crittenden and Judith Kimble. Imaging Sea Urchin Fertilization, Jon M. Holy. Imaging Immunolabelled Drosophila Embryos by Confocal Microscopy, James Langeland. Confocal Microscopy on Xenopus laevis Oocytes and Embryos, Denise L. Robb and Chris Wylie. Analyzing Morphogenetic Cell Behaviors in Vitally-Stained, Zebrafish Embryos, Mark S. Cooper, Leonard A. D'Amico, and Clarissa A. Henry. Confocal Imaging of Living Cells in Intact Embryos, Paul M. Kulesa and Scott E. Fraser. Live Confocal Analysis with Fluorescently Labeled Proteins, Helen Francis-Lang, Jonathan Minden, William Sullivan and Karen Oegema. Live Imaging with Green Fluorescent Protein, Jim Haseloff, Emma-Louise Dormand, and Andrea H. Brand. Fluorescent Calcium Indicators: Subcellular Behavior and Use in Confocal Imaging, Donald M. O'Malley, Barry J. Burbach, and Paul R. Adams. Intracellular pH and pCa Measurement, Michal Opas and Ewa Dziak. Measuring Dynamic Cell Volume In Situ by Confocal Microscopy, Rachel J. Errington and Nick S. White. Imaging Thick Tissues with Confocal Microscopy, Delphine Imbert, Janet Hoogstraate, Emmaline Marttin, and Chris Cullander. Measurement in the Confocal Microscope, Guy Cox. Presentation of Confocal Images, Georg Halder and Stephen Paddock. The Preparation of Stereoscopic 3D Illustrations of Confocal Data Sets for Publications and Slides, Gabriel G. Martins, Alan T. Stonebraker, and Robert G. Summers. Traditional Text-Based Databases and Image Gallery Databases, Harvey Karten. Morphing Confocal Images and Producing Digital Movies, Eric Hazen.Index.